Lumigen ECL Plus

Western Blotting HRP Substrate

Lumigen ECL Plus (PS-3) is an acridan-based technology chemiluminescent and chemifluorescent substrate for sensitive detection of Western blotted proteins that are bound to horseradish peroxidase (HRP) labeled antibodies.

  • Highly sensitive - approximately 5 pg per band of target protein on Western blot
  • Sustained luminescence - signal is sustained for over several hours
  • Dual signal functionality - chemiluminescent and chemifluorescent signals are generated simultaneously allowing detection on x-ray film or with a digital imager

Lumigen ECL Plus Technology

Lumigen ECL Plus utilizes a unique acridan based technology for the chemiluminescent and fluorescent detection of horseradish peroxidase (HRP) conjugates. The reaction involves the enzymatic generation of thousands of acridinium ester intermediates per minute which react with peroxide at alkaline pH to produce sustained high-intensity chemiluminescence. Additionally, the reaction end-product exhibits a long-lasting fluorescent signal which can be detected on a CCD imaging device.

western blot

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ECL Plus
Visit the Lumigen store to purchase Lumigen ECL Plus on-line.


Data Sheet: 
Application Instructions: 
Handling Instructions: 

Product Specifications

Horseradish Peroxidase (HRP)
Western Blotting
Low picogram to mid femtogram
Storage Conditions: 
2-8° C; Store in amber bottle to protect from light
2 years stored at 2-8° C
Working Solution: 
40:1 mix of A and B solutions

Ordering Information

Catalog Number: 
Lumigen ECL Plus