Dual Chemiluminescent/Fluorescent Substrates
Lumigen ECL Plus produces both a chemiluminescent and fluorescent signal in the presence of HRP with subattomole detection sensitivity. The following graph illustrates the comparable sensitivity attained via chemiluminescence or fluorescence. Fluorescent emission intensity was read at 515 nm.
This dual detection capability also allows for classic chemiluminescent blot detection on x-ray film or fluorescence detection in a digital imaging system.