Lumigen ECL Ultra (TMA-6)
Lumigen ECL Ultra (TMA-6) is a chemiluminescent substrate designed for the detection of HRP conjugates on membranes. It produces sustained, high-intensity luminescence for Western blots and related applications.
- Performance Profile: A high-sensitivity reagent for detecting horseradish peroxidase (HRP) activity. It generates long-lasting, bright signal ideal for digital imaging and film-based detection methods.
- Applications: Designed for life science researchers performing Western blots or immunoassays who require crisp, high-contrast protein detection.
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Lumigen ECL Ultra (TMA-6) Description
Lumigen ECL Ultra (TMA-6) is an ultra-sensitive chemiluminescent substrate formulated for the detection of horseradish peroxidase (HRP) conjugates on membranes. Designed for high-performance Western blotting applications, Lumigen ECL Ultra delivers exceptional signal intensity and sustained light output, enabling precise visualization of low-abundance protein targets.
Upon reaction with HRP-labeled antibodies, the substrate produces a rapid and highly luminous chemiluminescent signal. Its strong signal generation supports short exposure times while maintaining clarity and band definition. The sustained emission profile allows flexible imaging windows, supporting both digital imaging systems and film-based detection workflows.
Lumigen ECL Ultra is optimized for high-contrast detection across a broad dynamic range. It enables reliable visualization from nanogram to picogram protein levels, making it well suited for applications requiring enhanced sensitivity without sacrificing signal stability. The reagent supports crisp, well-defined band resolution and low background when used in properly optimized blotting systems.
Supplied as a two-component system (Solution A and Solution B) mixed in a 1:1 ratio to form the working substrate, Lumigen ECL Ultra reflects Lumigen’s decades of expertise in chemiluminescent chemistry and reagent formulation. It is trusted by research laboratories and assay developers seeking consistent, high-intensity detection performance.
Invented, developed, and manufactured by Lumigen, Lumigen ECL Ultra continues the company’s legacy of delivering reliable, high-sensitivity visible-light detection solutions for life science research.
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Lumigen ECL Ultra (TMA-6) Specifications
| Product Name | Lumigen® ECL Ultra (TMA-6) |
| Product Type | Chemiluminescent Reagent |
| Catalog Number | TMA-100, TMA-1000 |
| Contents | Solution A + Solution B; Mix 1:1 for working solution |
| Target Enzyme | Horseradish Peroxidase (HRP) |
| Primary Application | Ultra-sensitive detection of HRP-conjugated antibodies in Western blotting |
| Detection Method | Enhanced chemiluminescence (ECL) |
| Signal Characteristics | High intensity; Short exposure times (15–30 sec); Imaging possible for several hours |
| Assay Format | Western blot membrane-based detection |
| Working Solution Preparation | Mix Solutions A and B 1:1; Incubate membrane 2–3 minutes before imaging |
| Readout Equipment | X-ray film or CCD imager |
| Storage Conditions | 2–8°C; Protect from direct light; Do not freeze |
| Handling Requirements | Use in subdued light; Equilibrate to room temperature before mixing; Avoid membrane drying |
| Shelf Life | Stable when stored at 2–8°C protected from light (per manufacturer guidelines) |
| Container Requirements (If Repackaged) | New opaque HDPE or PP plastic containers only |
| Repackaging Guidance | Repackaging discouraged to prevent contamination and elevated background |
Why Choose Lumigen Chemiluminescent Reagents?
Lumigen is chemiluminescent technology leader, providing innovative reagents that bring unmatched sensitivity and reliability to clinical diagnostics and life science research.
Superior Quality
ISO 9001:2015–certified manufacturing in Southfield, Michigan ensures rigorous quality standards, reproducibility, and regulatory compliance.
Proven Consistency
Long-Term Stability
Formulations are designed to maintain signal integrity over time, supporting extended shelf life and dependable performance.